reversed phase high performance liquid chromatographic method for determination of quetiapine fumarate in pharmaceutical formulation and in spiked human urine

Authors

kanakapura b vinay jss college of arts, commerce & sciene

hosakere d. revanasiddappa

nagaraju rajendraprasad

pavagada j. ramesh

abstract

a precise and feasible reversed-phase high-performance liquid chromatographic method for the determination of an antipsychotic drug quetiapine fumarate (qtf) in pharmaceuticals and spiked human urine sample has been developed and validated. the analysis was carried out using a ods (250 mm × 4.6 mm i.d., 5 µm particle size) chromatopack column. mobile phase containing a mixture of acetonitrile and 0.1% phosphate buffer (ph 3.1) (40:60) was pumped at a flow rate of 1 ml/min with uv detection at 240 nm at ambient column temperature (25 ºc). the method showed good linearity in the range of 0.09 – 18 µg/ml qtf with limits of detection (lod) and quantification (loq) values of 0.03 and 0.09 µg/ml, respectively. the suggested method was successfully applied for the analysis of qtf in bulk drug, tablets and human urine with average recoveries of 100.06, 100.26 and 98.83%, respectively. the intra- and inter-day rsd values were less than 5%. the method is accurate, precise, sensitive and selective for routine analysis in quality control laboratories.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

High performance liquid chromatographic method for determination of ezetimibe in pharmaceutical formulation tablets

Ezetimide belongs to a class of lipid lowering    compounds    that    selectively    inhibits intestinal   absorption   of   cholesterol   and   related phytosterols. The purpose of this study is to establish a reliable and quick method for the assignment of ezetimibe in tablets form b...

full text

A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin

Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

full text

A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin

Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

full text

high performance liquid chromatographic method for determination of ezetimibe in pharmaceutical formulation tablets

ezetimide belongs to a class of lipid lowering compounds that selectively inhibits intestinal absorption of cholesterol and related phytosterols. the purpose of this study is to establish a reliable and quick method for the assignment of ezetimibe in tablets form by high performance liquid chromatography with ultraviolet detection (hplc-uv). a rapid and sensitive hplc method has been developed ...

full text

A reversed-phase high-performance liquid chromatographic method for the determination of zafirlukast in pharmaceutical formulations and human plasma.

Zafirlukast (ZAF) is a leukotriene receptor antagonist used in the treatment of chronic asthma. In this study, a simple and sensitive reversed-phase, high-performance liquid chromatographic method was developed for the determination of ZAF in pharmaceutical formulations and human plasma. Piribedil was used as an internal standard. Analysis was carried out on a Nucleosil C18 100 A (150 mm x 4.6 ...

full text

My Resources

Save resource for easier access later


Journal title:
journal of reports in pharmaceutical sciences

جلد ۲، شماره ۲، صفحات ۱۳۱-۱۳۹

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023